Expanding the toolbox for nanoparticle trapping and spectroscopy with holographic optical tweezers

We have developed a workstation based on holographic tweezers to optically trap, move and characterize metal nanoparticles. Our advanced darkfield imaging system allows us to simultaneously image and take spectra of single trapped metal nanoparticles. We take advantage of the beamshaping abilities of the spatial light modulator and correct for aberrations of the trapping optics. We monitor the improvement of the optical trap with video-based nanoparticle tracking. Furthermore we theoretically assess the capabilities and limitations of video-based tracking for nanoparticle position detection, in particular with respect to acquisition frequencies below the corner frequency

On the dragging of light by a rotating medium

When light is passing through a rotating medium the optical polarization is rotated. Recently, it has been reasoned that this rotation applies also to the transmitted image. We examine these two phenomena by extending an analysis of Player (Player 1976 Proc. R. Soc. A 349, 441-445) to general electromagnetic fields. We find that in this more general case, the wave equation inside the rotating medium has to be amended by a term which is connected to the orbital angular momentum (OAM) of the light. We show that optical spin and OAM account for the rotation of the polarization and the rotation of the transmitted image, respectively

Optical orbital angular momentum

We present a brief introduction to the orbital angular momentum of light, the subject of our theme issue and, in particular, to the developments in the 13 years following the founding paper by Allen et al. (Allen et al. 1992 Phys. Rev. A 45, 8185 (doi:10.1103/PhysRevA.45.8185)). The papers by our invited authors serve to bring the field up to date and suggest where developments may take us next

Mathieu beams as versatile light moulds for 3D micro particle assemblies

We present tailoring of three dimensional light fields which act as light moulds for elaborate particle micro structures of variable shapes. Stereo microscopy is used for visualization of the 3D particle assemblies. The powerful method is demonstrated for the class of propagation invariant beams, where we introduce the use of Mathieu beams as light moulds with non-rotationally-symmetric structure. They offer multifarious field distributions and facilitate the creation of versatile particle structures. This general technique may find its application in micro fluidics, chemistry, biology, and medicine, to create highly efficient mixing tools, for hierarchical supramolecular organization or in 3D tissue engineering

The effect of external forces on discrete motion within holographic optical tweezers

Holographic optical tweezers is a widely used technique to manipulate the individual positions of optically trapped micron-sized particles in a sample. The trap positions are changed by updating the holographic image displayed on a spatial light modulator. The updating process takes a finite time, resulting in a temporary decrease of the intensity, and thus the stiffness, of the optical trap. We have investigated this change in trap stiffness during the updating process by studying the motion of an optically trapped particle in a fluid flow. We found a highly nonlinear behavior of the change in trap stiffness vs. changes in step size. For step sizes up to approximately 300 nm the trap stiffness is decreasing. Above 300 nm the change in trap stiffness remains constant for all step sizes up to one particle radius. This information is crucial for optical force measurements using holographic optical tweezers

Generalised photon sieves: fine control of complex fields with simple pinhole arrays

Spatial shaping of light beams has led to numerous new applications in fields such as imaging, optical communication, and micromanipulation. However, structured radiation is less well explored beyond visible optics, where methods for shaping fields are more limited. Binary amplitude filters are often used in these regimes and one such example is a photon sieve consisting of an arrangement of pinholes, the positioning of which can tightly focus incident radiation. Here, we describe a method to design generalized photon sieves: arrays of pinholes that generate arbitrary structured complex fields at their foci. We experimentally demonstrate this approach by the production of Airy and Bessel beams, and Laguerre–Gaussian and Hermite–Gaussian modes. We quantify the beam fidelity and photon sieve efficiency, and also demonstrate control over additional unwanted diffraction orders and the incorporation of aberration correction. The fact that these photon sieves are robust and simple to construct will be useful for the shaping of short- or long-wavelength radiation and eases the fabrication challenges set by more intricately patterned binary amplitude masks

Speeding up liquid crystal SLMs using overdrive with phase change reduction

Nematic liquid crystal spatial light modulators (SLMs) with fast switching times and high diffraction efficiency are important to various applications ranging from optical beam steering and adaptive optics to optical tweezers. Here we demonstrate the great benefits that can be derived in terms of speed enhancement without loss of diffraction efficiency from two mutually compatible approaches. The first technique involves the idea of overdrive, that is the calculation of intermediate patterns to speed up the transition to the target phase pattern. The second concerns optimization of the target pattern to reduce the required phase change applied to each pixel, which in addition leads to a substantial reduction of variations in the intensity of the diffracted light during the transition. When these methods are applied together, we observe transition times for the diffracted light fields of about 1 ms, which represents up to a tenfold improvement over current approaches. We experimentally demonstrate the improvements of the approach for applications such as holographic image projection, beam steering and switching, and real-time control loops

Increasing trap stiffness with position clamping in holographic optical tweezers

We present a holographic optical tweezers system capable of position clamping multiple particles. Moving an optical trap in response to the trapped object's motion is a powerful technique for optical control and force measurement. We have now realised this experimentally using a Boulder Nonlinear Systems Spatial Light Modulator (SLM) with a refresh rate of 203Hz. We obtain a reduction of 44% in the variance of the bead's position, corresponding to an increase in effective trap stiffness of 77%. This reduction relies on the generation of holograms at high speed. We present software capable of calculating holograms in under 1ms using a graphics processor unit. © 2009 Optical Society of America

Violation of Leggett inequalities in orbital angular momentum subspaces

We report an experimental test of Leggett's non-local hidden variable theory in an orbital angular momentum (OAM) state space of light. We show that the correlations we observe are in conflict with Leggett's model, thus excluding a particular class of non-local hidden variable theories for the first time in a non-polarization state space. It is known that the violation of the Leggett inequality becomes stronger as more detection settings are used. The required measurements become feasible in an OAM subspace, and we demonstrate this by testing the inequality using three and four settings. We observe excellent agreement with quantum predictions and a violation of five and six standard deviations, respectively, compared to Leggett's non-local hidden variable theory